Pingfang Tian头像

Pingfang Tian

Professor

Research direction: metabolic engineering, synthetic biology, and biopesticides

100029

Education: PhD

10 Access

  • Email: tianpf@mail.buct.edu.cn
  • Office :

Introduction

Pingfang received Phd degree from Zhejiang University in 2003. Then he joined Beijing University of Chemical Technology (BUCT) as a faculty. He was promoted as a professor in 2011. Since then he has served as a doctoral supervisor for 13 years.

Education

Work Experience

Social Position

Social Activities

Research

Research interests comprise (1)microbial metabolic engineering for biomanufacturing, (2)synthetic biology for strain improvement, (3)biopesticides, (4)others fostering innovation.


Teaching

Biochemistry Experiments (for undergraduate)

Molecular Biology and Gene Manipulation (for postgraduate)

Advanced Molecular Biology (for PHD candidate)



Postgraduates

Funding

Ongoing projects:

National Natural Science Foundation of China (22278022)

National Key Research and Development Program of China (2023YFA0914700)



Vertical Project

Horizontal Project

Publications

1) CRISPR/Cas13 technology portfolio and alliance with other tools. Biotechnology Advances, 2022,108047. doi: 10.1016/j.biotechadv.2022.108047. (IF=17.681corresponding author)

2)Delineating biosynthesis of Huperzine A, a plant-derived medicine for the treatment of Alzheimer's disease. Biotechnol Advances, 2022, 60:108026. doi: 10.1016/j.biotechadv.2022.108026. (IF=17.681corresponding author)

3)Charting the landscape of RNA polymerases to unleash their potential in strain improvement. Biotechnology Advances, 2022, 54:107792. doi: 10.1016/j.biotechadv.2021.107792. (IF=14.227corresponding author)

4)Genomic landscapes of bacterial transposons and their applications in strain improvement. Appl Microbiol Biotechnol, 2022, 106(19-20):6383-6396. (IF=5.56corresponding author)

5)Rethinking biosynthesis of Aclacinomycin A. Molecules 2023, 28, 2761. https://doi.org/10.3390/molecules28062761 (IF=4.927corresponding author)

6)Switching metabolic flux by engineering tryptophan operon-assisted CRISPR interference system in Klebsiella pneumoniae. Metabolic Engineering, 2021, 65, 30-41(IF=9.783, corresponding author)

7)Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria. World J Microbiol Biotechnol, 2021, 37(7): 117 (corresponding author)

8)Engineering glucose to glycerol pathway in Klebsiella pneumoniae and boosting 3-hydroxypropionic acid production through CRISPR interference. Front Bioeng Biotechnol, 2022, 10: 908431. doi: 10.3389/fbioe.2022.908431(corresponding author)

9)A rapid liquid chromatography-electrospray ionization-ion mobility spectrometry method for monitoring nine representative metabolites in the seedlings of cucumber and wheat. Journal of Separation Science, 2021, 44(3): 709-716 (IF=3.614, corresponding author)

10)Intensifying niacin-based biosynthesis of NAD+ to enhance 3-hydroxypropionic acid production in Klebsiella pneumoniae. Biotechnol Lett, 2021, 43(1): 223-234  (corresponding author)

11)Development of T7 expression system in Klebsiella pneumoniae. Biotechnology and Bioengineering, 2020, 117, 2446-2459 (corresponding author)

12)Engineering CRISPR interference system to repress Class 1 integron in E. coli. Antimicrob Agents Chemother, 2020, 64(3): e01789-19 (corresponding author)

13)CRISPR interference-guided modulation of glucose pathways to boost aconitic acid production in Escherichia coli. Microbial Cell Factory, 2020, 19(1):174. doi: 10.1186/s12934-020-01435-9 (corresponding author)

14)Engineering CRISPR interference system to enhance the production of pyrroloquinoline quinone in Klebsiella pneumoniae. Lett Appl Microbiol, 2020, 71(3): 242-250 (corresponding author)

15)Improved production of pyrroloquinoline quinone by simultaneous augmentation of its synthesis gene expression and glucose metabolism in Klebsiella pneumoniae. Curr Microbiol, 2020, 77(7): 1174-1183 (corresponding author)

16)Exploiting tandem repetitive promoters for high-level production of 3-hydroxypropionic acid. Appl Microbiol Biotechnol, 2019, 103(10): 4017-4031 (corresponding author)

17)Physiological investigations of the influences of byproduct pathways on 3-hydroxypropionic acid production in Klebsiella pneumoniae. J Basic Microbiol, 2019, 59(12):1195-1207 (corresponding author)

18)Two-dimensional analysis of phenolic acids in seedling roots by high performance liquid chromatography-electrospray ionization-ion mobility spectrometry. Analytical Methods, 2019, 11, 610-617 (corresponding author)

19)Multipath effects of berberine on peach brown rot fungus Monilinia fructicola. Crop Protection, 2019, 116, 92-100 (corresponding author)

20)Development of cyclic AMP receptor protein-based artificial transcription factor for intensifying gene expression. Appl Microbiol Biotechnol, 2018, 102(4): 1673-1685 (corresponding author)

21)Engineering CRISPR interference system in Klebsiella pneumoniae for attenuating lactic acid synthesis. Microbial Cell Factories, 2018, 17(1): 56-67  (corresponding author)

22)Fundamental CRISPR-Cas9 tools and current applications in microbial systems. Systems and Synthetic Biology, 2017, 2(3): 219-225 (first author)

23)Evaluation of Berberine as a natural compound to inhibit peach brown rot pathogen Monilinia fructicola. Crop Protection, 2017, 91, 20-26 (corresponding author)

24)High production of 3-hydroxypropionic acid in Klebsiella pneumoniae by systematic optimization of glycerol metabolism. Scientific Reports, 2016, 6, 26932  (corresponding author)

25)Development of Red recombinase system and antisense RNA technology in Klebsiella pneumoniae for production of chemicals. RSC Advances, 2016, 6, 79920-79927  (corresponding author)

26)3-Hydroxypropionaldehyde-specific aldehyde dehydrogenase from Bacillus subtilis catalyzes 3-hydroxypropionic acid production in Klebsiella pneumoniae. Biotechnol Lett, 2015, 37(3): 717-724, 2015  (corresponding author)

27)Distinct promoters affect pyrroloquinoline quinone production in recombinant Escherichia coli and Klebsiella pneumoniae. Curr Microbiol, 2014, 69(4): 451-456  (corresponding author)

28)Engineered constitutive pathway in Klebsiella pneumoniae for 3-hydroxypropionic acid production and implications for decoupling glycerol dissimilation pathways. Curr Microbiol, 2013, 66(3): 293-299 (corresponding author)

29)Classifying the DNA assembly protocols for devising cellar architectures. Biotechnology Advances, 2011, 29(1): 156-163 (corresponding author)

30)Berberine as a natural compound inhibits the development of brown rot fungus Monilinia fructicola, Crop Protection, 2010, 29, 979-984 (corresponding author)

 


Awards

Patent

Honor Reward

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