Research
We conducted research to address the bottlenecks in the biosensors and liquid biopsy fields, including sensitivity, detection time, and detection cost. (1) Our team proposed and explored strategies such as single-molecule DNA dynamic hybridization molecular probes, significantly reducing the sample-to-answer time and background for single-molecule detection. This enables fast, non-amplification, and highly sensitive detection of various biomarkers, including nucleic acids, proteins, and small molecules. (2) We conducted in-depth research on the kinetics of DNA dynamic reaction networks and build a multi-detection system based on time-resolved signals. They make outstanding progress in the multiple, rapid, and low-cost detection of pathogenic nucleic acids.
Teaching
Biomedical Sensors, Medical Molecular Diagnostics, and Modern Medical Laboratory Technology.
Funding
项目编号 Project number | 项目名称 Project name | 经费(万元) Fund (RMB10,000) | 起止年月Duration | 项目来源 Source | 32271521 | Construction of DNA Timer Barcodes and Their Applications in Multiplex Detection and Multiplex In Situ Imaging. | 54 | 2023.01-2026.12 | National Natural Science Foundation of China General Project | 31971361 | Construction of Single-Molecule Kinetic Fingerprint Signals and Their Applications in Quantifying Circulating miRNA and Intracellular miRNA. | 61 | 2020.01-2023.12 | National Natural Science Foundation of China General Project | 31600687 | Research on Gene Mutation Detection Based on Nucleic Acid Transient Hybridization. | 20 | 2017.01-2019.12 | National Natural Science Foundation of China Youth Project | 2022YFC2603902 | Nano-Based New Technologies for Immune Protection Against Biothreat Pathogens. | 385 | 2022.12-2025.12 | Key Research Development Program of China | 2019YFC1200502 | Discovery of New Pathogens Carried by Important Vertebrate Vectors and Their Potential Pathogenicity Studies. | 230 | 2019.12-2022.12 | Key Research Development Program of China |
Publications
Shengnan Fu, Junjie Li, Jing Chen, Linghao Zhang, Jiajia Liu, Xin Su* Bacteriophage λ exonuclease and a 5′-phosphorylated DNA guide allow PAM-independent targeting of double-stranded nucleic acids, Nature Biotechnology, 2024, doi: 10.1038/s41587-024-02388-9 Linghao Zhang, Hongyang Zhao, Huixiao Yang, Xin Su* Coarse-grained model simulation-guided localized DNA signal amplification probe for miRNA detection, Biosensors & Bioelectronics, 2023, 239, 115622. Chunyi Zhang, Ziyan Zhou, Shengnan Fu, Changyuan Yu*, Muhammad Irfan*, Xin Su*, DNA nanoprobes for detection and imaging of glycoproteins, Nano Today, 2023, 51, 101893. Yingjie Yu, Linghao Zhang, Zhaohui Qin, Johannes Karges, Haihua Xiao*, Xin Su*, Unraveling and Overcoming Platinum Drug-Resistant Cancer Tumors with DNA Nanostructures, Advanced Functional Materials, 2023, 33, 2208797 Zhaohui Qin, Yu Liu, Linghao Zhang, Jiajia Liu, and Xin Su*, Programming Dissipation Systems by DNA Timer for Temporally Regulating Enzyme Catalysis and Nanostructure Assembly, ACS Nano, 2022, 16, 14274–14283. Qiaoni Kou, Lei Wang, Linghao Zhang, Liang Ma, Shengnan Fu, and Xin Su*, Simulation-Assisted Localized DNA Logical Circuits for Cancer Biomarkers Detection and Imaging, Small, 2022, 18, 2205191. Jing Chen, Shengnan Fu, Chunyi Zhang, Huiyu Liu,* and Xin Su*, DNA Logic Circuits for Cancer Theranostics, Small, 2022, 18, 2108008. Na Li, Yuee Zhao, Yu Liu, Zhe Yin, Rui Liu, Linghao Zhang, Liang Ma, Xiaochuan Dai, Dongsheng Zhou,* Xin Su*, Self-resetting molecular probes for nucleic acids detection enabled by fuel dissipative systems, Nano Today, 2021, 41, 101308. Yecan Pan, Rui Weng*, Linghao Zhang, Jing Qiu, Xinlu Wang, Guangqin Liao, Zhaohui Qin, Lingpu Zhang, Haihua Xiao, Yongzhong Qian*, Xin Su*, Simulation guided intramolecular orthogonal reporters for dissecting cellular oxidative stress and response, Nano Today, 2022, 46, 101573 Shengnan Fu, Tengfang Zhang, Huanling Jiang, Yan Xu, Jing Chen, Linghao Zhang, Xin Su*, DNA nanotechnology enhanced single-molecule sensing and imaging, Trends in Analytical Chemistry, 2021, 140, 116267 Yingnan Deng, Liang Ma, Qianqian Han, Changyuan Yu, Alexander Johnson-Buck,* and Xin Su*, DNA-Templated Timer Probes for Multiplexed Sensing, Nano Letters, 2020, 20, 2688-2694. Congshan Wang, Yingjie Yu, Muhammad Irfan, Bolong Xu, Junjie Li, Linghao Zhang, Zhaohui Qin, Changyuan Yu, Huiyu Liu,* and Xin Su*, Rational Design of DNA Framework-Based Hybrid Nanomaterials for Anticancer Drug Delivery, Small, 2020, 16, 2002578. Yi Zhang, Yingnan Deng, Congshan Wang, Lidan Li, Lida Xu, Yingjie Yu*, Xin Su*, Probing and Regulating the Activity of Cellular Enzyme by DNA Tetrahedron Nanostructures, Chemical Science, 2019, 10, 5959-5966. Lina Li, Yingjie Yu, Congshan Wang, Qianqian Han, Xin Su*, Transient Hybridization Directed Nanoflare for Single-Molecule miRNA Imaging, Analytical Chemistry, 2019, 91, 11122−11128. Alexander Johnson-Buck#, Xin Su#, Maria D Giraldez, Meiping Zhao, Muneesh Tewari, Nils G. Walter*, Kinetic fingerprinting to identify and count single nucleic acids, Nature Biotechnology, 2015, 33, 730-732.
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